Ahmed mansouri max planck biography

Cellular subtype identity in the pancreas.

Ahmed Mansouri Molecular Cel Differentiation ().

Ahmed Mansouri integrates many fields, such as Genetics and Anatomy, in his works.

Address correspondence to: Patrick Collombat or Ahmed Mansouri, Max Planck Institute for Biophysical Chemistry, Department of Molecular Cell.

In this work, rather than injuring the pancreas, we developed a mouse model allowing the inducible misexpression of the proendocrine gene Neurog3 in ductal.

Ahmed Mansouri integrates many fields, such as Genetics and Anatomy, in his works....

Embryonic endocrine pancreas and mature β cells acquire α and PP cell phenotypes upon Arx misexpression

Generation of transgenic animals conditionally misexpressing Arx.

The consequences of Arx and/or Pax4 loss-of-function mutations are consistent with antagonistic roles for Arx and Pax4 in supporting the α cell or the β/δ cell fate, respectively (24, 33). To gain further insight into the fate-specifying activities of Arx and Pax4 throughout pancreas morphogenesis, we took advantage of the Cre-LoxP system to generate transgenic mice capable of conditionally misexpressing the Arx gene (cArxOE mice).

The construct used consisted of the CMV enhancer upstream of the human β-actin promoter (CAG) controlling the constitutive expression of the eGFP gene flanked by LoxP sites (Figure 1, top). The Arx cDNA was cloned downstream of eGFP together with an IRES–β-galactosidase–encoding sequence.

With the use of pronuclear injection, 5 independent transgenic lines were established